Until they came into place, producers could not be prosecuted for failing to comply with NLIS.
Agriculture Department animal biosecurity director Ashley Mercy said WA had the most effective sheep and goat tracing system in Australia.
Dr Mercy said in the event of a disease or residue occurrence the ability to trace sheep had been significantly improved.
"The system also underpins the identification of sheep for live export and allows abattoirs to meet the requirements of a number of markets for traceability of sheep slaughtered," Dr Mercy said.
"The new regulations have been developed in consultation with all sections of the sheep and goat industry through the NLIS sheep and goats Implementation Working Group."
The regulations require producers to identify sheep and goats on the property of birth with a year colour ear tag imprinted with the owner¹s brand.
Purchased animals must be identified before re-sale or movement by applying an additional pink ear tag imprinted with the current owner's brand.
Before purchasing sheep or goats the buyer must also have a registered brand linked to the property where the stock are to be moved.
The brand or PIC must be provided to the sellers of the stock, who need to keep a record of the transaction.
The regulations allow exemptions for lambs moved directly from the property of birth to abattoirs, but some processors require that all lambs received be tagged.